Cellular pH gradient in tumor versus normal tissue: potential exploitation for the treatment of cancer.
Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research. Intracranial nonthermal irreversible electroporation: in vivo analysis. A parametric study delineating irreversible electroporation from thermal damage based on a minimally invasive intracranial procedure. Non-thermal irreversible electroporation for deep intracranial disorders. Direct therapeutic applications of calcium electroporation to effectively induce tumor necrosis. Nonthermal irreversible electroporation for intracranial surgical applications. In vivo results of a new focal tissue ablation technique: irreversible electroporation. Physical Properties of Tissue: A Comprehensive Reference Book. Theoretical analysis of the thermal effects during in vivo tissue electroporation. Tumour hypoxia induces a metabolic shift causing acidosis: a common feature in cancer. Electroporation of brain endothelial cells on chip toward permeabilizing the blood-brain barrier. Kinetics of sealing for transient electropores in isolated mammalian skeletal muscle cells. Rhabdomyolysis due to pulsed electric fields. Fundamentals of Heat and Mass Transfer (7th ed.). A three-dimensional in vitro tumor platform for modeling therapeutic irreversible electroporation. Tumor ablation with irreversible electroporation. Preclinical validation of electrochemotherapy as an effective treatment for brain tumors. The ability to ablate a larger volume with lowered energies would improve the efficacy and safety of IRE therapy.Īgerholm-Larsen, B., H. These findings, combined with our numerical modeling, suggest the ability to ablate up to 3.2× larger volumes of tissue in vivo when combining IRE and calcium. The calcium-induced death response of reversibly electroporated cells is confirmed by electrochemotherapy pulses which also induced cell death with calcium but not without. Adjuvant 5 mM CaCl 2 induces death in cells reversibly electroporated by IRE, thereby lowering the electric field thresholds required for cell death to nearly half that of IRE alone. We present evidence that IRE used with adjuvant calcium (5 mM CaCl 2) results in a nearly twofold increase in ablation area in vitro compared to IRE alone. For IRE to be used safely in the brain, methods are needed to extend the area of ablation without increasing applied voltage, and thus, thermal damage. Cells exposed to lower energies become temporarily permeable while greater energy exposure results in cell death.
IRE works by exposing tissues to pulsed electric fields which cause cell membrane disruption.
Irreversible electroporation (IRE) is a cellular ablation method used to treat a variety of cancers.
0 kommentar(er)
